4AU3
Crystal Structure of a Hsp47-collagen complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 101.260, 101.260, 366.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.740 - 2.780 |
| R-factor | 0.2044 |
| Rwork | 0.203 |
| R-free | 0.23380 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXE |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Rmerge | 0.100 | 0.100 |
| Number of reflections | 48652 | |
| <I/σ(I)> | 18.4 | 1.6 |
| Completeness [%] | 99.1 | 94.7 |
| Redundancy | 8.2 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
