4AU2
Crystal Structure of a Hsp47-collagen complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.498, 92.784, 100.458 |
| Unit cell angles | 95.24, 98.95, 96.73 |
Refinement procedure
| Resolution | 98.620 - 2.300 |
| R-factor | 0.2044 |
| Rwork | 0.203 |
| R-free | 0.24040 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXE |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.500 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.030 | 0.730 |
| Number of reflections | 70114 | |
| <I/σ(I)> | 18.2 | 1.3 |
| Completeness [%] | 96.6 | 9 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
