4ATO
New insights into the mechanism of bacterial Type III toxin-antitoxin systems: selective toxin inhibition by a non-coding RNA pseudoknot
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-28 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 6 |
| Unit cell lengths | 127.100, 127.100, 37.735 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.950 - 2.200 |
| R-factor | 0.1618 |
| Rwork | 0.160 |
| R-free | 0.19170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xdb |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.083 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 2.230 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.050 | 0.550 |
| Number of reflections | 34618 | |
| <I/σ(I)> | 13.6 | 4.6 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 11 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | PRECIPITANT CONTAINS 0.2 M AMMONIUM PHOSPHATE, 0.1 M TRIS-HCL PH 8.5, 50% MPD. CRYSTALLIZATION WITH A SITTING DROP, VAPOUR DIFFUSION METHOD. |
