4AT7

Crystal structure of the NF90-NF45 dimerisation domain complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-05-10
Detector	DECTRIS PILATUS 6M
Spacegroup name	I 2 2 2
Unit cell lengths	82.900, 131.300, 176.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	88.000 - 1.902
R-factor	0.1863
Rwork	0.185
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	INITIAL MODEL GENERATED FROM A GOLD DERIVATIVE SOLVED IN SPACE GROUP C 2
RMSD bond length	0.007
RMSD bond angle	1.035
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.000	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.080	0.600
Number of reflections	74599
<I/σ(I)>	15.3	3.4
Completeness [%]	98.4	95.4
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		100 MM MES PH 6.0, 0.22 M SODIUM MALONATE, 18 % PEG 8000