4ASZ
Crystal structure of apo TrkB kinase domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-07 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 86.993, 96.777, 46.378 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.540 - 1.700 |
R-factor | 0.181 |
Rwork | 0.178 |
R-free | 0.20330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1luf |
RMSD bond length | 0.008 |
RMSD bond angle | 0.940 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | BUSTER (2.9.7) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.300 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.070 | 0.390 |
Number of reflections | 43721 | |
<I/σ(I)> | 14.9 | 3.5 |
Completeness [%] | 99.7 | 100 |
Redundancy | 4.5 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 277 | THE APO-TRKB PROTEIN WAS CONCENTRATED TO 6 MG/ML AND CRYSTALLIZED IN 3 M NA-FORMATE, PH 8.0 AT 4 DEGREES C. |