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4ASZ

Crystal structure of apo TrkB kinase domain

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE ID14-2
Synchrotron siteESRF
BeamlineID14-2
Temperature [K]100
Detector technologyCCD
Collection date2007-11-07
DetectorADSC CCD
Spacegroup nameP 21 21 2
Unit cell lengths86.993, 96.777, 46.378
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution10.540 - 1.700
R-factor0.181
Rwork0.178
R-free0.20330
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1luf
RMSD bond length0.008
RMSD bond angle0.940
Data reduction softwareMOSFLM
Data scaling softwareSCALA
Phasing softwareMOLREP
Refinement softwareBUSTER (2.9.7)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]42.3001.790
High resolution limit [Å]1.7001.700
Rmerge0.0700.390
Number of reflections43721
<I/σ(I)>14.93.5
Completeness [%]99.7100
Redundancy4.54.5
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
18277THE APO-TRKB PROTEIN WAS CONCENTRATED TO 6 MG/ML AND CRYSTALLIZED IN 3 M NA-FORMATE, PH 8.0 AT 4 DEGREES C.

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