4ASG
The structure of modified benzoquinone ansamycins bound to yeast N- terminal Hsp90
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-07 |
Detector | RIGAKU CCD |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 74.126, 74.126, 110.177 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 74.130 - 2.200 |
R-factor | 0.19489 |
Rwork | 0.193 |
R-free | 0.24072 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | IN-HOUSE MODEL |
RMSD bond length | 0.020 |
RMSD bond angle | 2.224 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.130 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.070 | 0.460 |
Number of reflections | 16272 | |
<I/σ(I)> | 21.6 | 2.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 20.01 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | pH 7 |