4ASB
The structure of modified benzoquinone ansamycins bound to yeast N- terminal Hsp90
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-28 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 74.460, 74.460, 110.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.650 - 3.080 |
| R-factor | 0.17786 |
| Rwork | 0.173 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IN-HOUSE MODEL |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.882 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.650 | 3.160 |
| High resolution limit [Å] | 3.080 | 3.080 |
| Rmerge | 0.150 | 0.660 |
| Number of reflections | 6071 | |
| <I/σ(I)> | 8.6 | 2.2 |
| Completeness [%] | 98.9 | 100 |
| Redundancy | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
