4ARV
Yersinia kristensenii phytase apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.410, 67.723, 73.164 |
| Unit cell angles | 76.67, 87.35, 78.32 |
Refinement procedure
| Resolution | 27.920 - 1.670 |
| R-factor | 0.16538 |
| Rwork | 0.164 |
| R-free | 0.19847 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ars |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.805 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 1.750 | 1.750 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.050 | 0.180 |
| Number of reflections | 118484 | |
| <I/σ(I)> | 24.9 | 4.9 |
| Completeness [%] | 90.8 | 79.1 |
| Redundancy | 5.6 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | PROTEIN CONCENTRATION 10MG/ML; CONDITION A12 FROM PACT SCREEN: SUCCINATE PHOSPHATE BORATE PH 5.0, 25% PEG1500 |
