4ART
STRUCTURE OF THE ORF273 PROTEIN FROM THE ACIDIANUS TWO-TAILED VIRUS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2010-07-25 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 78.620, 78.620, 189.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.420 - 2.150 |
R-factor | 0.2041 |
Rwork | 0.202 |
R-free | 0.23380 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | autoSHARP |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.210 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.080 | 0.740 |
Number of reflections | 32549 | |
<I/σ(I)> | 24 | 3.72 |
Completeness [%] | 97.9 | 99.9 |
Redundancy | 12.8 | 13.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | PROTEIN SOLUTION AT 5 MG/ML IN 10 MM BICINE PH 8.5 AND 100 MM NACL WAS MIXED IN A 3:1 RATIO TO A SOLUTION CONTAINING 3.6% ISOPROPANOL AND 1.9 M AMMONIUM SULPHATE AS PRECIPITANT AGENTS IN 5 MM MGCL2 AND 2 MM AMP | ||
1 | 6 | PROTEIN SOLUTION AT 5 MG/ML IN 10 MM BICINE PH 8.5 AND 100 MM NACL WAS MIXED IN A 3:1 RATIO TO A SOLUTION CONTAINING 3.6% ISOPROPANOL AND 1.9 M AMMONIUM SULPHATE AS PRECIPITANT AGENTS IN 5 MM MGCL2 AND 2 MM AMP |