4ARH
X ray structure of the periplasmic zinc binding protein ZinT from Salmonella enterica
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-22 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 58.534, 58.534, 289.316 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.690 - 2.300 |
R-factor | 0.22388 |
Rwork | 0.221 |
R-free | 0.28841 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 10EK |
RMSD bond length | 0.012 |
RMSD bond angle | 1.283 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.110 | 0.240 |
Number of reflections | 14007 | |
<I/σ(I)> | 25.62 | 14.75 |
Completeness [%] | 99.1 | 99.9 |
Redundancy | 12.7 | 14.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | AMMONIUM SULFATE 2 M, SODIUM ACETATE 0.1 M PH 5 |