4ARE
Crystal structure of the collagenase Unit of collagenase G from Clostridium histolyticum at 2.19 angstrom resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-24 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.290, 108.660, 181.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.500 - 2.190 |
| R-factor | 0.19567 |
| Rwork | 0.193 |
| R-free | 0.24297 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y3u |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.314 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.500 | 2.310 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.070 | 0.600 |
| Number of reflections | 56974 | |
| <I/σ(I)> | 10.7 | 2 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 4.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.25 | 22.5% PEG 3350, 0.15M TRISODIUMCITRATE PH 8.2 |
