4AR9
Crystal structure of the peptidase domain of collagenase T from Clostridium tetani at 1.69 angstrom resolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-09-11 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.890, 102.110, 104.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.450 - 1.690 |
R-factor | 0.17482 |
Rwork | 0.172 |
R-free | 0.22105 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2y3u |
RMSD bond length | 0.012 |
RMSD bond angle | 1.393 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.850 | 1.780 |
High resolution limit [Å] | 1.690 | 1.690 |
Rmerge | 0.100 | 0.800 |
Number of reflections | 88975 | |
<I/σ(I)> | 8.7 | 2 |
Completeness [%] | 96.2 | 94.5 |
Redundancy | 5.7 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 25% PEG 3350, 100MM NACL, 0.1M MES PH 6.5 |