4AQY

Structure of ribosome-apramycin complexes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Collection date	2009-04-25
Spacegroup name	P 41 21 2
Unit cell lengths	402.180, 402.180, 175.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 3.500
R-factor	0.1934
Rwork	0.193
R-free	0.23510
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1j5e
RMSD bond length	0.007
RMSD bond angle	1.203
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.600
High resolution limit [Å]	3.500	3.500
Rmerge	0.100	1.100
Number of reflections	178694
<I/σ(I)>	16	2
Completeness [%]	99.7	99.5
Redundancy	6.9	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	MPD, NH4CL, KCL, CACL2, MAGNESIUM ACETATE, SODIUM CACODYLATE, PH 6.5, VAPOR DIFFUSION, HANGING DROP AT 277K