4AQB
MBL-Ficolin Associated Protein-1, MAP-1 aka MAP44
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-11 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 94.150, 94.150, 242.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.360 - 4.200 |
| R-factor | 0.2797 |
| Rwork | 0.279 |
| R-free | 0.30060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3DEM AND 3GOV |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.400 | 4.360 |
| High resolution limit [Å] | 4.200 | 4.200 |
| Rmerge | 0.280 | 0.560 |
| Number of reflections | 7844 | |
| <I/σ(I)> | 8.7 | 2.8 |
| Completeness [%] | 93.5 | 95.2 |
| Redundancy | 3.64 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2 M CALCIUM ACETATE, 0.1 M SODIUM CACODYLATE PH 6.5, 40% W/V PEG 400 |
