4APZ
Structure of B. subtilis genomic dUTPase YncF in complex with dU, PPi and Mg in P1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-19 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 97.060, 97.070, 193.850 |
| Unit cell angles | 89.72, 88.47, 90.11 |
Refinement procedure
| Resolution | 36.440 - 2.010 |
| R-factor | 0.17122 |
| Rwork | 0.169 |
| R-free | 0.21185 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xcd |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.902 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.440 | 2.060 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.090 | 0.400 |
| Number of reflections | 434755 | |
| <I/σ(I)> | 5.9 | 2.4 |
| Completeness [%] | 96.7 | 95.9 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 30% MPD, 0.1 M SODIUM ACETATE PH 4.6, 0.02 M CACL2 |
