4APV
The Klebsiella pneumoniae primosomal PriB protein: identification, crystal structure, and ssDNA binding mode
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2010-01-20 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 64.773, 36.934, 39.451 |
| Unit cell angles | 90.00, 113.77, 90.00 |
Refinement procedure
| Resolution | 26.649 - 2.095 |
| R-factor | 0.2052 |
| Rwork | 0.198 |
| R-free | 0.27360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v1q |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.001 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.400 | 2.100 |
| High resolution limit [Å] | 2.100 | 2.090 |
| Rmerge | 0.060 | 0.260 |
| Number of reflections | 5031 | |
| <I/σ(I)> | 40.7 | 6.4 |
| Completeness [%] | 95.6 | 99.8 |
| Redundancy | 3.4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 50% ETHANOL, 10 MM SODIUM ACETATE |
