4APO
AIP TPR domain in complex with human Tomm20 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-09 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 60.200, 106.820, 68.470 |
| Unit cell angles | 90.00, 100.85, 90.00 |
Refinement procedure
| Resolution | 28.454 - 1.895 |
| R-factor | 0.1836 |
| Rwork | 0.181 |
| R-free | 0.23440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4aif |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.906 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER (FOR MR) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.520 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.040 | 0.130 |
| Number of reflections | 32055 | |
| <I/σ(I)> | 18.5 | 8.7 |
| Completeness [%] | 96.3 | 90.9 |
| Redundancy | 3.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | PEG 3350, AMMONIUM SULFATE, BIS-TRIS PH 5.5 |
