4AOW
Crystal structure of the human Rack1 protein at a resolution of 2.45 angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2011-10-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 134.260, 134.260, 135.186 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.870 - 2.450 |
| R-factor | 0.1941 |
| Rwork | 0.193 |
| R-free | 0.21420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dm0 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.180 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.630 | 2.590 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.110 | 0.750 |
| Number of reflections | 45262 | |
| <I/σ(I)> | 15.4 | 2.5 |
| Completeness [%] | 98.8 | 92.1 |
| Redundancy | 9.3 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | pH 8.5 |
