4AOM
MTIP and MyoA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | RIGAKU SATURN 944 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.264, 54.704, 75.024 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 13.218 - 1.939 |
| R-factor | 0.1788 |
| Rwork | 0.177 |
| R-free | 0.22310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qac |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.968 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.910 | 2.040 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.210 | 0.150 |
| Number of reflections | 11754 | |
| <I/σ(I)> | 10.8 | 5.4 |
| Completeness [%] | 98.7 | 94.5 |
| Redundancy | 4.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2M POTASSIUM THIOCYANATE, 20% PEG3350, pH 7.5 |
