4AOB
SAM-I riboswitch containing the T. solenopsae Kt-23 in complex with S- adenosyl methionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-28 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 61.300, 61.300, 157.538 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.950 |
| R-factor | 0.1914 |
| Rwork | 0.189 |
| R-free | 0.23943 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gx5 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.545 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.060 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.110 | 0.460 |
| Number of reflections | 6810 | |
| <I/σ(I)> | 26.8 | 3.05 |
| Completeness [%] | 98.5 | 89.5 |
| Redundancy | 9.5 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 40 MM NA-CACODYLATE PH 7, 80 MM KCL, 30 MM BACL2, 12 MM SPERMINE HCL 8%(V/V) MPD |
