4AO5
B. subtilis prophage dUTPase YosS in complex with dUMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-28 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 99.820, 100.010, 99.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.650 - 1.600 |
| R-factor | 0.15347 |
| Rwork | 0.150 |
| R-free | 0.21365 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xcd |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.446 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.800 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.080 | 0.600 |
| Number of reflections | 131522 | |
| <I/σ(I)> | 7.8 | 2.1 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 5.9 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 14% PEG 3350, 0.1 M SODIUM MALONATE, 0.1 M, BIS-TRIS PROPANE PH 7.5 |
