4AO4
Structural Determinants of the beta-Selectivity of a Bacterial Aminotransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-30 |
| Detector | MARRESEARCH SX-165 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 184.387, 94.864, 102.973 |
| Unit cell angles | 90.00, 113.77, 90.00 |
Refinement procedure
| Resolution | 47.430 - 1.950 |
| R-factor | 0.18498 |
| Rwork | 0.183 |
| R-free | 0.21631 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.380 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.070 | 0.240 |
| Number of reflections | 116568 | |
| <I/σ(I)> | 7.6 | 3.3 |
| Completeness [%] | 98.7 | 99.6 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
