4AMS

A Megaviridae ORFan gene encode a new nucleotidyl transferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	105
Detector technology	CCD
Collection date	2011-11-18
Detector	ADSC CCD
Spacegroup name	P 43 21 2
Unit cell lengths	100.632, 100.632, 168.822
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.558 - 2.600
R-factor	0.213
Rwork	0.211
R-free	0.24680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4amq
RMSD bond length	0.009
RMSD bond angle	1.338
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CaspR
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.600	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.090	0.480
Number of reflections	26030
<I/σ(I)>	6.3
Completeness [%]	99.8	99.8
Redundancy	8.2	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		PROTEIN CONCENTRATION: 5 TO 12 MG/ML IN 10 MM CAPS BUFFER, 150 MM NACL, PH 10.5 RESERVOIR SOLUTION: 150 MM NA ACETATE PH 6