4AMQ
A Megaviridae Orfan gene encodes a new nucleotidyl transferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2010-11-07 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 60.445, 91.092, 152.994 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.290 - 2.170 |
| R-factor | 0.2206 |
| Rwork | 0.219 |
| R-free | 0.24420 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.678 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.400 | 2.250 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rmerge | 0.080 | 0.360 |
| Number of reflections | 20963 | |
| <I/σ(I)> | 3.8 | 1.9 |
| Completeness [%] | 96.0 | 96 |
| Redundancy | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | PROTEIN CONCENTRATION: 10 MG/ML IN 10 MM CHES BUFFER PH 9.0 RESERVOIR: 4 TO 12% PEG 4000, 0.1 M SODIUM CACODYLATE BETWEEN PH 6 AND 7, 0.1 M MNCL2 |
