4AM7
ADP-BOUND C-TERMINAL DOMAIN OF ACTIN-RELATED PROTEIN ARP8 FROM S. CEREVISIAE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-02 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 137.718, 89.795, 149.647 |
| Unit cell angles | 90.00, 114.37, 90.00 |
Refinement procedure
| Resolution | 19.810 - 3.250 |
| R-factor | 0.22259 |
| Rwork | 0.219 |
| R-free | 0.28417 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4am6 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.566 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.430 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.090 | 0.440 |
| Number of reflections | 25357 | |
| <I/σ(I)> | 8.5 | 2.5 |
| Completeness [%] | 96.4 | 94.6 |
| Redundancy | 3.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 19.5% W/V PEG 5000 MME, 0.32 M LI2SO4, 0.1 M MES PH 6.5, 0.002 M ADP, 0.005 M MGCL2 |
