4AM0
Structure of Dengue virus strain 4 DIII in complex with Fab 2H12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 69.930, 92.800, 96.590 |
| Unit cell angles | 118.55, 90.33, 104.05 |
Refinement procedure
| Resolution | 41.990 - 3.020 |
| R-factor | 0.1988 |
| Rwork | 0.196 |
| R-free | 0.25220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.250 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.000 | 3.100 |
| High resolution limit [Å] | 3.020 | 3.020 |
| Rmerge | 0.110 | 0.480 |
| Number of reflections | 38472 | |
| <I/σ(I)> | 8.1 | 1.8 |
| Completeness [%] | 96.0 | 96.2 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 20% PEG 3350, 0.2 M POTASSIUM THIOCYNATE, pH 7.5 |
