4AKX
Structure of the heterodimeric complex ExoU-SpcU from the type III secretion system (T3SS) of Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-12-11 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 154.887, 52.799, 121.811 |
Unit cell angles | 90.00, 127.18, 90.00 |
Refinement procedure
Resolution | 97.050 - 2.940 |
R-factor | 0.21102 |
Rwork | 0.208 |
R-free | 0.26437 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.012 |
RMSD bond angle | 1.291 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 97.050 | 3.120 |
High resolution limit [Å] | 2.940 | 2.940 |
Rmerge | 0.090 | 0.670 |
Number of reflections | 17008 | |
<I/σ(I)> | 13.1 | 3 |
Completeness [%] | 98.1 | 91.6 |
Redundancy | 6.4 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 9 | 50 MM BICINE PH 9.0, 10% PEG 4000 |