4AKC
Structure of Galactose Binding lectin from Champedak (CGB) with Gal(beta)1,3-GalNac
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-08 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 76.287, 121.673, 77.563 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.490 - 2.300 |
| R-factor | 0.2005 |
| Rwork | 0.198 |
| R-free | 0.24590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ak4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.150 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.610 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.200 | 0.830 |
| Number of reflections | 32825 | |
| <I/σ(I)> | 17.1 | 3.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.3 | 13.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.6 | 40% PEG 600, 100 MM PHOSPHATE/CITRATE BUFFER PH 4.2 |
