4AJX
Ligand controlled assembly of hexamers, dihexamers, and linear multihexamer structures by an engineered acylated insulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.310, 62.490, 57.270 |
| Unit cell angles | 90.00, 111.80, 90.00 |
Refinement procedure
| Resolution | 40.497 - 1.200 |
| R-factor | 0.1415 |
| Rwork | 0.141 |
| R-free | 0.15960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1evr |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.614 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE: DEV_947)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.500 | 1.230 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.036 | 0.410 |
| Number of reflections | 90966 | |
| <I/σ(I)> | 19.15 | 2.51 |
| Completeness [%] | 92.4 | 60.3 |
| Redundancy | 4.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.6 M IMIDAZOLE/MALONIC ACID PH 7.0 |
