4AJT
The crystal structure of mouse protein-Z dependent protease inhibitor(mZPI)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Collection date | 2012-02-02 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 83.530, 83.530, 181.260 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 72.340 - 2.500 |
| R-factor | 0.23089 |
| Rwork | 0.229 |
| R-free | 0.26894 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f1s |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.052 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.340 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.270 | 1.060 |
| Number of reflections | 23621 | |
| <I/σ(I)> | 4.9 | 1.6 |
| Completeness [%] | 98.0 | 94.4 |
| Redundancy | 5.3 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 4.3 M NACL,0.1 M HEPES-NA, PH7.2 |
