4AJ5
Crystal structure of the Ska core complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-06-17 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 174.400, 193.121, 198.267 |
| Unit cell angles | 90.00, 95.21, 90.00 |
Refinement procedure
| Resolution | 69.031 - 3.320 |
| R-factor | 0.2368 |
| Rwork | 0.234 |
| R-free | 0.27970 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.281 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.800 | 3.500 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.060 | 0.750 |
| Number of reflections | 91708 | |
| <I/σ(I)> | 5.4 | 1.2 |
| Completeness [%] | 94.7 | 94.1 |
| Redundancy | 1.7 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.6 | 50 MM TRIS PH 7.6, 0.2 M LITHIUM ACETATE, 45% MPD |
