4AJ0
Crystallographic structure of an amyloidogenic variant, 3rCW, of the germinal line lambda 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-01 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 31 |
| Unit cell lengths | 41.807, 41.807, 171.035 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.631 - 1.700 |
| R-factor | 0.1765 |
| Rwork | 0.174 |
| R-free | 0.21160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lil |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.135 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.060 | 0.430 |
| Number of reflections | 36831 | |
| <I/σ(I)> | 10.2 | 1.8 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 5.7 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3 | 1.6 M AMMONIUM SULFATE, 40 % TREHALOSE, pH 3 |
