4AIR

Leishmania major cysteine synthase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-17
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	48.958, 86.325, 134.019
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 1.800
R-factor	0.15921
Rwork	0.157
R-free	0.20826
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bhs
RMSD bond length	0.015
RMSD bond angle	1.438
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0119)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.900	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.070	0.490
Number of reflections	53553
<I/σ(I)>	18	3.5
Completeness [%]	100.0	100
Redundancy	6	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1