4AH6

Human mitochondrial aspartyl-tRNA synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-08-21
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 1 2 1
Unit cell lengths	142.400, 82.600, 146.300
Unit cell angles	90.00, 100.40, 90.00

Refinement procedure

Resolution	29.916 - 3.700
R-factor	0.2222
Rwork	0.219
R-free	0.28010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	HOMOLOGY MODEL BASED ON PDB ENTRY 1C0A
RMSD bond length	0.005
RMSD bond angle	1.084
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.000	3.800
High resolution limit [Å]	3.700	3.700
Rmerge	0.180	0.640
Number of reflections	35768
<I/σ(I)>	9.4	2.1
Completeness [%]	98.8	91.2
Redundancy	7.2	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5		SITTING DROPLETS WERE PREPARED BY MIXING 0.5 UL HSADRS2 AT 10.6 MG/ML (IN 50 MM HEPES-NA PH 7.5, 150 MM NACL, 0.1 MM EDTA, 10% (V/V) GLYCEROL AND 1 MM DTT) WITH 0.5 UL BOTTOM PHASE OF A BIPHASIC MIXTURE OF 50 MM BIS-TRIS PH 5.5, 20% (M/V) PEG-3350 AND 1 M AMMONIUM SULFATE AND EQUILIBRATED BY VAPOR DIFFUSION OVER 50 UL OF THE LATTER SOLUTION.