4AH3
Crystal structure of the holo omega-transaminase from Chromobacterium violaceum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.890, 62.230, 119.610 |
| Unit cell angles | 75.14, 81.73, 76.17 |
Refinement procedure
| Resolution | 115.190 - 1.570 |
| R-factor | 0.225 |
| Rwork | 0.222 |
| R-free | 0.27322 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qj3 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.459 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.730 | 1.660 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.100 | 0.300 |
| Number of reflections | 218828 | |
| <I/σ(I)> | 4.4 | 1.9 |
| Completeness [%] | 94.4 | 90.9 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
