4AGV
Structure of a tetrameric galectin from Cinachyrella sp. (Ball sponge)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 170 |
| Detector technology | CCD |
| Collection date | 2010-02-21 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.960, 66.510, 71.550 |
| Unit cell angles | 117.07, 95.39, 99.56 |
Refinement procedure
| Resolution | 35.610 - 2.650 |
| R-factor | 0.20789 |
| Rwork | 0.203 |
| R-free | 0.30341 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1is5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.515 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.700 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.130 | 0.530 |
| Number of reflections | 15419 | |
| <I/σ(I)> | 5.8 | 1.6 |
| Completeness [%] | 85.8 | 85.7 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.2 M CACL2, 0.1 M TRIS PH 8.5, 20% PEG4000 |
