4AGG
Structure of a tetrameric galectin from Cinachyrella sp. (Ball sponge)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 170 |
| Detector technology | CCD |
| Collection date | 2010-03-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 61.005, 126.635, 80.436 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.220 - 2.980 |
| R-factor | 0.19042 |
| Rwork | 0.186 |
| R-free | 0.27490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1is5 SIDECHAINS TRUNCATED |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.837 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.750 | 3.050 |
| High resolution limit [Å] | 2.980 | 2.980 |
| Rmerge | 0.150 | 0.450 |
| Number of reflections | 6308 | |
| <I/σ(I)> | 11.7 | 2.1 |
| Completeness [%] | 95.6 | 74.4 |
| Redundancy | 8.7 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.2 M LITHIUM SULFATE, 0.1 M TRIS PH 8.5, 30% PEG3000 |
