4AG2
Human Chymase - Fynomer Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-06-02 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.937, 64.124, 174.987 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.750 - 1.800 |
| R-factor | 0.18021 |
| Rwork | 0.179 |
| R-free | 0.21168 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IN HOUSE STRUCTURE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.356 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.870 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.090 | 0.670 |
| Number of reflections | 62210 | |
| <I/σ(I)> | 10.95 | 1.49 |
| Completeness [%] | 99.7 | 99 |
| Redundancy | 6.19 | 5.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.15 M DL-MALIC ACID PH 7.0, 20% PEG 3350 |
