4AFU
Human Chymase - Fynomer Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-05-22 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.992, 59.057, 158.079 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.276 - 1.820 |
| R-factor | 0.1898 |
| Rwork | 0.188 |
| R-free | 0.22520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IN HOUSE STRUCTURE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.004 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.910 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.090 | 0.630 |
| Number of reflections | 50464 | |
| <I/σ(I)> | 9.72 | 1.48 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 6.13 | 5.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2 M SODIUM CHLORIDE, 0.1 M BIS-TRIS PH 6.5, 25% PEG 3350 |
