4AFS
Human Chymase - Fynomer Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-05-07 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 78.584, 89.072, 48.494 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.490 - 1.900 |
| R-factor | 0.18913 |
| Rwork | 0.187 |
| R-free | 0.23796 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IN HOUSE STRUCTURE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.511 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.070 | 0.670 |
| Number of reflections | 35077 | |
| <I/σ(I)> | 10.68 | 1.51 |
| Completeness [%] | 99.6 | 97.5 |
| Redundancy | 6.43 | 6.35 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.2 M AMMONIUM ACETATE, 0.1 M TRIS PH 8.5, 25 % PEG 3350 |
