4AEN
HLA-DR1 with covalently linked CLIP106-120 in reversed orientation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 66.196, 145.111, 108.777 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.414 - 2.200 |
| R-factor | 0.1853 |
| Rwork | 0.183 |
| R-free | 0.22070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pdo |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.053 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.420 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.080 | 0.560 |
| Number of reflections | 27008 | |
| <I/σ(I)> | 15.8 | 2.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2 M (NH4)2SO4, 0.1 M TRIS, PH 8.0 |
