4AEK
Structural and biochemical characterization of a novel Carbohydrate Binding Module of endoglucanase Cel5A from Eubacterium cellulosolvens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793, 0.97934, 0.9769 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 47.859, 47.859, 191.598 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.450 - 1.750 |
| R-factor | 0.1902 |
| Rwork | 0.188 |
| R-free | 0.22712 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.076 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | SAS (AUTORICKSAW) |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.870 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.070 | 0.320 |
| Number of reflections | 13971 | |
| <I/σ(I)> | 10.4 | 1.7 |
| Completeness [%] | 99.1 | 94.8 |
| Redundancy | 5.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 292 | 0.23 M AMMONIUM SULFATE, 0.1 M SODIUM ACETATE PH 4.6, 26%(M/V) PEG 2K MME GROWN AT 292 K, USED 30% GLYCEROL AS CRYOPROTECTANT |
