4AEH
Crystal structure of the anti-AaHI Fab9C2 antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-10 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.930, 71.030, 65.960 |
| Unit cell angles | 90.00, 111.82, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.600 |
| R-factor | 0.17606 |
| Rwork | 0.174 |
| R-free | 0.21359 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.377 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.650 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.060 | 0.420 |
| Number of reflections | 63057 | |
| <I/σ(I)> | 11.26 | 2.8 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 12.5% PEG 4000, 0.1 M IMIDAZOLE-MALATE PH 7.0, 50 MM NACL |
