4ADZ
Crystal Structure of the apo form of a Copper-sensitive operon Regulator (CsoR) protein from Streptomyces lividans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-24 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 41.630, 54.550, 91.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.550 - 1.700 |
| R-factor | 0.18754 |
| Rwork | 0.185 |
| R-free | 0.22858 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.663 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.900 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.058 | 0.280 |
| Number of reflections | 22220 | |
| <I/σ(I)> | 12.1 | 2.1 |
| Completeness [%] | 93.8 | 71.7 |
| Redundancy | 3.1 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 1.26 M AMMONIUM SULPHATE, 0.1 M SODIUM CITRATE PH 4 |
