4ADP
HCV-J6 NS5B POLYMERASE V405I MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-11 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.500, 95.600, 114.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.105 - 1.902 |
| R-factor | 0.1729 |
| Rwork | 0.171 |
| R-free | 0.20090 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2xwh |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.008 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX (REFINE) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.110 | 1.100 |
| Number of reflections | 56181 | |
| <I/σ(I)> | 13.2 | 2 |
| Completeness [%] | 99.7 | 98.3 |
| Redundancy | 6 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 2.17% PEG 4000, 0.05 M TRI-SODIUM CITRATE, PH 6.5 |
