4ADI
Crystal structure of the Rubella virus envelope glycoprotein E1 in post-fusion form (crystal form I)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2007-11-02 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 129.980, 121.380, 126.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.1682 |
| Rwork | 0.167 |
| R-free | 0.18310 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.960 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.300 | 1.870 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.090 | 0.500 |
| Number of reflections | 188417 | |
| <I/σ(I)> | 16.7 | 2 |
| Completeness [%] | 98.3 | 88.1 |
| Redundancy | 8.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1-3% PEG 4K, 0.1M NAHEPES PH7.5-8, 30% GLYCEROL |
