4ABX
Crystal structure of Deinococcus radiodurans RecN coiled-coil domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-01 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.210, 43.970, 133.630 |
| Unit cell angles | 90.00, 97.71, 90.00 |
Refinement procedure
| Resolution | 45.933 - 2.041 |
| R-factor | 0.2033 |
| Rwork | 0.201 |
| R-free | 0.25020 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.730 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.500 | 2.150 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.070 | 0.320 |
| Number of reflections | 52532 | |
| <I/σ(I)> | 8.4 | 2.8 |
| Completeness [%] | 97.1 | 98.2 |
| Redundancy | 2.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M HEPES PH 7, 30% PEG 6000, 1 M LICL 3% 1,2,3-HEPTANETRIOL. |
