4ABL
HUMAN PARP14 (ARTD8, BAL2) - MACRO DOMAIN 3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-01-26 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.705, 41.066, 108.918 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.790 - 1.150 |
R-factor | 0.16222 |
Rwork | 0.161 |
R-free | 0.19132 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4abk |
RMSD bond length | 0.017 |
RMSD bond angle | 1.589 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.180 |
High resolution limit [Å] | 1.150 | 1.150 |
Rmerge | 0.050 | 0.430 |
Number of reflections | 53461 | |
<I/σ(I)> | 20.9 | 3.9 |
Completeness [%] | 97.8 | 82.7 |
Redundancy | 6.7 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 30% PEGMME2000, 0.05M POTASSIUM BROMIDE |