4AAY
Crystal Structure of the arsenite oxidase protein complex from Rhizobium species strain NT-26
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 70 |
| Detector technology | CCD |
| Collection date | 2011-07-11 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 149.130, 232.960, 141.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 141.870 - 2.700 |
| R-factor | 0.19433 |
| Rwork | 0.193 |
| R-free | 0.21128 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g8j |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.393 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER (FOR MR) |
| Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.100 | 0.370 |
| Number of reflections | 135790 | |
| <I/σ(I)> | 18 | 6.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.2 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M HEPES SODIUM PH 7.5, 2% PEG 400, 2.0 M AMMONIUM SULPHATE, 10% GLYCEROL |
