4AA6
The oestrogen receptor recognizes an imperfectly palindromic response element through an alternative side-chain conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.677, 113.093, 62.363 |
| Unit cell angles | 90.00, 117.45, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.600 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.193 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.800 |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.070 |
| Number of reflections | 22960 |
| Completeness [%] | 99.5 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20 MM MES PH 5.75, 1.8 MM SPERMINE, 2 MICROMOLAR ZINC CHLORIDE, 30 MM SODIUM CHLORIDE, 12 MM CALCIUM CHLORIDE, 10% MPD |
